Artemin ELISA Assay Kit
The Artemin ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 2 pg/mL
Dynamic Range: 30 – 1920 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL
Alternative Names: Human Artemin, hARTN
The kit should be stored at +2…+6°C. The unopened kit is stable until the expiry date, printed on the kit label. The expiry date of each unopened component is printed on the label of the individual component. After opening, the components should be used within 8 weeks (microwell plate desiccation recommended).
As hARTN may be unstable in low concentrations (diluted standard stock solution, standards and diluted samples) it is recommended to prepare the standards and samples directly before the test procedure. hARTN standards can be stored at -20°C up to one week if frozen immediately after preparation. Thaw and use only once.
The hARTN (Human Artemin) test is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with hARTN-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and hARTN molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated hARTN-specific monoclonal detection antibodies bind to the same epitope on another molecule of dimeric hARTN. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the colour produced is directly proportional to the amount of hARTN in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.