Anti-ZnT8 ELISA Assay Kit
Anti-ZnT8 ELISA Assay Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 10 U/ml
Dynamic Range: 10 – 500 U/ml
Incubation Time: overnight
Sample Type: Serum, Plasma
Sample Size: 25 µL
Alternative Names: Human Zinc Transporter 8 ELISA, Zinc Transporter 8 ELISA
For Research Use Only
Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to environmental agents. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80 – 90% of the cells are lost. This process may take years to complete and may occur at any time in all ages.
During the preclinical phase, this autoimmune process is marked by circulating autoantibodies to beta cell antigens. These autoantibodies, such as anti-insulin (IAA), anti-glutamic acid decarboxylase (GAD), anti-tyrosine phosphatase ICA 512 (IA2) and zinc transporter 8 (ZnT8), are present years before the onset of type 1 diabetes and prior to clinical symptoms.
ZnT8 autoantibodies are directed principally to the C terminal domain of ZnT8 (residues 268 – 369). Human population gene polymorphism at the codon for the 325th amino acid results in the expression of three protein variants: Arginine (R) 325, Tryptophan (W) 325 and very rarely Glutamine (Q) 325. ZnT8 autoantibodies may be specific to the R 325 or W 325 variant, or may be residue 325 non-specific. Sera that react with the Q allele only are extremely rare. The Eagle Biosciences anti-ZnT8 ELISA Assay kit is capable of detecting, and quantifying, autoantibodies specific to R 325 or to W 325, or to residue 325 non-specific variants.