Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is Developed and Manufactured by Generic Assays
Size: 96 wells (24 samples x 4)
Incubation Time: 2 hours
Sample Type: Serum or Plasma
Number Of Samples Per Kit: 24 (22 samples + controls)
Sample Size: 50 µl
For Research Use Only. Not for use in diagnostic procedures.
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is a reagent kit for the determination of IgG antibodies against immunodominant major antigens of the SARS coronavirus-2. The test kit consists of modules separately coated with the major antigens of the virus:
The samples are pipetted horizontally over the 4 strips of a module (e.g. A1+A2+A3+A4), any antibodies present react with the specific antigens bound to the solid phase in the first reaction step. Unbound sample components are removed by a wash step after 45 minutes incubation at 37 °C.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 45 min at 37 °C, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the added colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature (18-25 °C) turning the solution from blue to yellow.
The optical density (OD) of the solution measured at 450 nm is directly proportional to the amount of specific antibodies bound.
Specimen collection and storage
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma samples (citrate, EDTA, heparin) can be used too, too. Hyperlipemic, hemolytic or contaminated samples must not be used and the samples must not contain preservatives.
Samples can be stored up to 5 days at 2 – 8 °C in the primary tubes. For longer storage, sera or plasmas extracted from the primary tubes must be frozen at -20°C. Repeated freezing and thawing should be avoided. If necessary, aliquots should be prepared before freezing.
Samples are diluted during the Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA test, controls should not be diluted.
Coronavirus COVID-19 IgG ELISA Assay
Coronavirus COVID-19 IgM ELISA Assay Kit
Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously known as “2019-nCoV) is a zoonotic single-stranded RNA viruses with positive polarity, belonging to the coronaviruses family. It is classified in the genus beta-coronavirus, which also includes SARS-CoV (2003) and MERS-CoV (2012). Among the coronavirus structural proteins envelope, membrane, spike and the nucleocapsid, the last two are the main immunogens.
An infection with the SARS-CoV-2 can lead to a respiratory syndrome called Corona Virus Disease 2019 (COVID-19). It was emerging in human living since the end of 2019 in the province of Hubei, China, and rapidly spreading with a pandemic trend all over the word.
People infected with SARS-CoV-2 may remain asymptomatic or develop only mild upper airways symptoms, similar to those of a cold or flu. Others develop pneumonia and ARDS requiring intubation in ICU, and may undergo complications that can be fatal. It can take up to 14 days after exposure to SARS-CoV-2 before they appear. Currently suitable methods for SARS-CoV-2 diagnosis, examine the genetic material of the virus in oral swabs using the polymerase chain reaction (PCR). But PCR only shows a positive result if the virus is still present. The tests cannot identify individuals who have gone through an infection, recovered and have the virus from their bodies removed.
Enzyme immunoassays, on the other hand, are serological tests that allow the determination of specific antibodies to SARS-CoV-2. For a significant serological result, 2 samples from one host should be tested, one sample at the onset of symptoms and a second sample collected about 4 weeks later.
- Bring all reagents and the required number of test cavities to room temperature (18-25 °C) before use. Mix gently without causing foam.
- Add 50 µl of Start reagent (G) to all wells.
- Dispense into 4 horizontal wells of one module: 200 µl diluted samples. Alternatively 200 µl of Negative control (N) and Positive control
(P) instead of 2 sample
- Cover plate, shake for 30 seconds, incubate 45 minutes at 37 °C.
- Decant, then wash each well 5 times using 350 µl wash solution (made of B), use a soak time of 20 seconds each.
- Add 100 µl of conjugate (D) solution to all wells.
- Cover plate, incubate 45 minutes at 37 °C.
- Repeat wash step 5.
- Add 100 µl of substrate (E) to each well.
- Incubate 15 min protected from light at room temperature (18…25 °C).
- Add 100 µl of stop solution (F) to each well and mix gently.
- Read the OD at 450 nm versus 620 (630 nm) within 20 min after adding the stop solution.