Anti-Aprotinin IgG Antibodies ELISA


The Anti-Aprotinin IgG Antibodies ELISA Assay Kit is designed for the quantitative determination of IgG Aprotinin Antibodies in serum samples. The Anti-Aprotinin Antibodies ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.

SKU: APR31-K01 Categories: , ,

Anti-Aprotinin IgG Antibodies ELISA

The Anti-Aprotinin IgG Antibodies ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 12.5 U/ml
Dynamic Range: 12.5 U/ml – 200 U/ml
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 5 µL
Controls Included

Assay Background

Aprotinin is used in operations to reduce intra-operative and postoperative bleeding tendency. Aprotinin as an allogeneic protein has antigenic properties. It has been shown that formation of IgG antibodies to aprotinin takes place in about 50% of all treated patients. During aprotinin reexposure allergic reactions may occur due to these preformed anti-aprotinin antibodies. Lethal anaphylactic shocks have been described in literature. This Anti-Aprotinin Antibodies Assay Kit allows the detection of IgG antibodies against aprotinin in human serum.

Related Products

Anti Angiotensin II Receptor 1 IgG4 Antibody ELISA Assay Kit
AT1R IgG2 Antibody ELISA

Additional Information

Assay Principle

Aprotinin has been pre-coated onto a microtiter plate. During incubation the anti-aprotinin antibodies are immobilized on the plate. For detection of bound antibodies an enzyme-linked anti-human-IgG anti­body conjugate is added. After washing off any unbound conjugate, a substrate solution is added. The color developing correlates to the amount of bound antibody conjugate. Absorption at 450 nm is proportional to the concentration and/or avidity of anti-aprotinin antibodies.

Assay Procedure

  1. Prepare all reagents and samples as directed in the previous section.
  2. Pipette 100 µl of diluted samples, standards, controls or diluent (as blank) into the wells.
  3. Seal wells with adhesive strip and incubate for 1 hour at room temperature.
  4. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
  5. Dispense 100 µl of diluted HRP conjugate into each well
  6. Seal wells with adhesive strip and incubate for 30 minutes at room temperature.
  7. Repeat the wash as in step 4.
  8. Dispense 100 µl of TMB substrate solution into each well.
  9. Incubate for 15 minutes at room temperature in the dark.
  10. Add 100 µl of stop solution to each well.
  11. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Typical Standard Curve

Anti-Aprotinin IgG Antibodies ELISA


Product Manual

Product Citations