Anti-AChR ELISA Assay Kit
Anti-AChR ELISA Assay Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 0.23 nmol/l
Dynamic Range: 0.2 – 20 nmol/l
Incubation Time: Overnight
Sample Type: Serum
Sample Size: 100 µl
Alternative Names: Anti-Acetylcholine Receptor ELISA, Human Anti-AChR ELISA
For Research Use Only
The Anti-AChR ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of autoantibodies to acetylcholine receptor (AChR Abs) in human serum.
The assay system depends on the ability of AChR Abs in human serum to bind to similar sites on the AChR as various monoclonal antibodies such as MAb1 (coated on ELISA plate wells) and/or MAb2 and/or MAb3 (which are labeled with biotin). In the absence of AChR Abs a complex is formed between MAb1 coated on the plate wells, the AChR and MAb2 and MAb3 biotin. MAb2 and MAb3 biotin bound are then detected by addition of streptavidin peroxidase (SA-POD), substrate (TMB) and stop solution. In the presence of AChR Abs the formation of the MAb1-AChR-MAb2/MAb3 biotin complex is inhibited, resulting in less SA-POD being bound and a reduction in final absorbance at 450nm. The higher the concentration of AChR Abs in the test serum the greater the inhibition of MAb biotin binding.