Alpha Amylase Saliva Assay Kit

$240.00

The Eagle Biosciences α-Amylase Saliva Assay Kit is a kinetic colorimetric method for quantitative determination of α-amylase in saliva. The Alpha-Amylase Saliva Assay Kit is for research use only and not to be used for diagnostic procedure.

SKU: DCM075 Categories: , ,

Alpha Amylase Saliva Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 2.5 U/ml
Dynamic Range: 2.5 – 400 U/mL
Incubation Time: 3 minutes
Sample Type: Saliva
Sample Size: 10 µl

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Amylase is the name given to enzymes that break down starch. They are classified as saccharidases, enzymes that cleave polysaccharides.Although the amylases are designated by different greek letters, they all act on α-1,4 glycosidic bonds. The α-amylases are calcium metalloenzymes, completely unable to function in the absence of calcium. The α-amylase breaks down long-chain carbohydrates, ultimately yielding maltotriose and maltose from amylose, or maltose, glucose and “limit dextrin” from amylopectin. In animals, it is a major digestive enzyme.

Although found in many tissues, the α-amylase is most prominent in pancreatic juice and saliva which each have their own isoform. Salivary α-amylase breaks starch down into maltose and dextrin. This form is also called ptyalin. Ptyalin will break large, insoluble starch molecules into soluble starches (amylodextrin, erythrodextrin, achrodextrin) producing successively smaller starches and ultimately maltose. Ptyalin acts on linear α(1,4) glucosidic linkages, but compound hydrolysis requires an enzyme which acts on branched products. Salivary amylase is inactivated in the stomach by gastric acid. Pancreatic α-amylase randomly cleaves the α(1-4)glycosidic linkages of amylose to yield dextrin, maltose or glucose molecules.

Assay Principle

In the α-Amylase Saliva Assay Kit, the human α-Amylase hydrolyses the 2-chloro-4 nitrophenyl-α-maltotrioside (CNP-G3) in glucose polymers and short-chain p-nitrophenyl-oligosaccharide with formation of 2-chloro-4-nitrophenol (CNP).

The increase of the extinction is evaluated spectrophotometrically at 405 nm and is proportional to α-amylase activity in the sample.

Manual

Product Manual