Aggrecanase Sensitive Activity Assay Kit

$870.00

The Eagle Biosciences Aggrecanase Sensitive Activity Assay Kit measures activities of aggrecanases in the pM-concentration range. This high sensitivity is achieved with an engineered aggrecanase substrate derived from aggrecan interglobular domain. The Sensitive Aggrecanase Activity Assay allows measurement of aggrecanase activity in serum-free cell culture supernatants.

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Aggrecanase Sensitive Activity Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 12pM
Dynamic Range: 0.56 – 100 pM
Incubation Time: 4 hours
Sample Type: cell culture
Sample Size: 5 µL

Additional Information

Assay Background


Aggrecan is a large aggregating proteoglycan of articular cartilage [1]. It is found also in aorta tissue, discs, tendons [1] and in the perineuronal net [2]. The aggrecan core protein consists of 2317 amino acids [3]. Up to 130 glucosaminoglycan chains are attached to the core protein and the total molecular mass can reach 2.2 – 3.0 x 106 Daltons [4] Aggrecan-IGD-s (provided in this kit) is incubated with standard Aggrecanase (ADAMTS4) and samples of unknown aggrecanase activity. In addition to aggrecanase, different concentrations of aggrecanase inhibitors can be added. The reaction is stopped by dilution with EDTA-containing buffer.

Aggrecan is a large aggregating proteoglycan of articular cartilage [1]. It is found also in aorta tissue, discs, tendons [1] and in the perineuronal net [2]. The aggrecan core protein consists of 2317 amino acids [3]. Up to 130 glucosaminoglycan chains are attached to the core protein and the total molecular mass can reach 2.2 – 3.0 x 106 Daltons [4].

Typical Standard Curve


Manual

Product Manual