Aggrecanase Activity Assay Kit

$780.00

The Eagle Biosciences Aggrecanase Activity Assay Kit measures activity of aggrecanases. It is used to screen and characterize aggrecanase inhibitors. It consists of two modules: Aggrecanase module and the ELISA module. In this Assay a recombinant fragment of human aggrecan interglobular domain (aggrecan-IGD) is first digested with aggrecanase (ADAMTS4). The proteolytic cleavage of the substrate releases an aggrecan peptide with the N-terminal sequence ARGSVIL (ARGSVIL-peptide). The ARGSVIL-peptide-s is then quantified with two monoclonal anti-peptide antibodies. The Eagle Biosciences Aggrecanase Activity Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: 30 510 111 Categories: , ,

Aggrecanase Activity Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 12pM
Dynamic Range: 0.024 – 1.5 nM
Incubation Time: 3.5 hours
Sample Type: Cell culture supernatants
Sample Size: 5 µL

Additional Information

Assay Background

Aggrecan is a large aggregating proteoglycan of articular cartilage [1]. It is found also in aorta tissue, discs, tendon [1] and in the perineuronal net [2]. The aggrecan core protein consists of 2317 amino acids [3]. Up to 130 glucosaminoglycan chains are attached to the core protein and the total molecular mass can reach 2.2 – 3.0 x 106 daltons [4].

Assay Principle

Aggrecan-IGD (provided with this kit) is incubated with standard aggrecanase (ADAMTS4) and samples of unknown aggrecanase activity. In addition to aggrecanase, different concentrations of aggrecanase inhibitors can be added. The reaction is stopped by dilution with EDTA-containing buffer.

Typical Standard Curve

Manual

Product Manual