Aflatoxin Ochratoxin Zearalenon Immunoaffinity Column
The Aflatoxin Ochratoxin Zearalenon Immunoaffinity Column is For Research Use Only
Size: 3ml, 10 columns
This instruction of simultaneous Aflatoxin, Ochratoxin and Zearalenon (AOZ) determination in food and feed focuses on the enrichment step of extract using IAC and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods of Aflatoxin, Ochratoxin and Zearalenon determination in food and feed show low sensitivity because of interfering substances if problematic matrices are applied.
This method of content determination of Aflatoxins, Ochratoxins and Zearalenon combines the high selectivity of an IAC with its potential to concentrate eluate and additional step of purification by HPLC column.
Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. The given apparatus (e.g. HPLC system) might serve as example among other possibilities.
Extraction (Literature method given):
Samples which content of Aflatoxin, Ochratoxin and Zearalenon are to be analyzed, e.g. wheat, maize etc., are extracted by the method of Krska et al.1 using the solvent mixture of acetonitrile/water/acetic acid (79/20/1 v/v/v). E.g. to 25g of ground wheat kernels are added a volume of 100ml of the extraction solvent and processed as cited.