6 keto Prostaglandin F1 Alpha ELISA Assay Kit

$500.00

The Eagle Biosciences 6-keto-Prostaglandin F1 Alpha ELISA Assay kit is for the quantitative determination of  6-keto-Prostaglandin F1 Alpha in biological fluids by a microplate enzyme immunoassay (ELISA).

SKU: 6KP39-K01 Categories: , ,

6 keto Prostaglandin F1 Alpha ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.02 ng/ml
Dynamic Range: 0.02 – 20 ng/ml
Incubation Time: 2 hour
Sample Type: Biological Fluids
Sample Size: 1 mL

Product manufactured in the USA

Additional Information

Assay Background

6-keto-Prostaglandin F1a (6-k-PGF1a) is a stable hydrolyzed product of unstable prostacyclin (PGI2). PGI2 is derived from PGH2, which in turn, is synthesized from the cyclooxygenase pathway. Through this pathway it is the major product produced by the endothelial cells, although it is also synthesized in other cell types such as macrophages. Prostacyclin inhibits platelet aggregation and induces vasodilation. Quantitation of PGI2 production can be made by determining the level of 6-k-PGF1a.

Assay Principle

This 6-keto-Prostaglandin F1 Alpha ELISAAssay kit (Enzyme-Linked Immunosorbent Assay) is for the quantitative analysis of 6-keto-Prostaglandin F1 Alpha levels in biological fluid.  This test kit operates on the basis of competition between the enzyme conjugate and the 6-k-PGF1a in the sample for a limited number of binding sites on the antibody coated plate.

The sample or standard solution is first added to the microplate.  Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour.  During the incubation, competition for binding sites is taking place.  The plate is then washed to remove all of the unbound material.  The bound enzyme conjugate is detected by the addition of a substrate that generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the sample wells against the standard wells with a microplate reader at 650 nm.  The extent of color development is inversely proportional to the amount of 6-k-PGF1a in the sample or standard.  For example, the absence of 6-k-PGF1a in the sample will result in a bright blue color, whereas the presence of 6-k-PGF1a will result in decreased or no color development.

  1. Add 50 µL of Standards or Samples (may require diluting) to the corresponding wells on the microplate in duplicate.
  2. Add 50 µL of diluted 6-k-PGF1a-HRP Conjugate to each well. Incubate at room temperature for one hour.
  3. Wash the plate three times with 300 µL of diluted Wash Buffer per well. Wash 5 times if using an automated plate washer.
  4. Add 150 µL of TMB Substrate to each well. Incubate at room temperature for 30 minutes.
  5. Read the plate at 650 nm.

Alternately, the color reaction can be stopped after 10-15 minutes by adding 50 µL of 1 N HCl and read at 450 nm.

Cross Reactivity Data

6-keto-Prostaglandin F1a

100.0%

15-k-Prostaglandin F2a

0.66%

Prostaglandin F1a

76.77%

13, 14-d-15-k-prostaglandin F2a

0.48%

2,3-dinor-6-keto-Prostaglandin F1a

64.48%

Prostaglandin A2

0.30%

6-keto-Prostaglandin E1

49.92%

Prostaglandin A1

0.25%

Prostaglandin F2a

29.94%

Thromboxane B2

0.15%

Prostaglandin E2

11.84%

Prostaglandin B2

0.02%

Prostaglandin D2

8.36%

Prostaglandin B1

0.02%

11-d-Thromboxane B2

1.07%

Leukotriene B4

0.01%

Manual

Product Manual