21-Hydroxylase Autoantibodies ELISA Assay


The 21-Hydroxylase Autoantibodies ELISA Assay is intended for the quantitative determination of autoantibodies to 21-OH in human serum. The 21-Hydroxylase Autoantibodies ELISA Assay is intended for research use only and not for use in diagnostic or clinical procedures.

21-Hydroxylase Autoantibodies ELISA Assay

21-Hydroxylase Autoantibodies ELISA Assay Developed and Manufactured by Medipan

Size: 1×96 wells
Sensitivity: 0.13 U/ml
Standard Range: 0.01 – 100 U/ml
Incubation Time: Overnight + 100 minutes
Sample Type: Serum
Sample Size: 50 µl
Alternative Names: 21-OH Ab
For Research Use Only

Specimen collection and storage
Blood is taken by venipuncture. After clotting, the serum is separated by centrifugation. Do not use lipaemic or hemolytic serum samples. Do not use plasma samples in the test. The samples may be kept at 2 – 8 °C up to three days. Long-term storage requires storage at- 20 °C. Repeated freezing and thawing should be avoided. For multiple use, initially aliquot samples and store at -20 °C.

Assay Principle

The assay utilizes the ability of 21-OH Abs to act divalently, and to form a bridge between immobilized 21-OH and 21-OH-Biotin in the fluid phase. In the first step, 21-OH Ab present in samples binds with 21-OH immobilized onto the microtiter plate. In the second step, 21-OH-Biotin binds to this complex. The amount of 21-OH-Biotin bound correlates with the level of antibodies present in patient samples. Unbound 21-OH-Biotin is then removed by washing. Bound 21-OH-Biotin can then be quantified by addition of streptavidin peroxidase (SA-POD) and a colorigenic substrate (TMB), and reading the optical density at 450nm.

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Additional Information

Assay Background

Autoimmune destruction of the adrenal cortex is the most common cause of Addison’s disease and autoantibodies to corticosteroid producing cells in adrenal cortex. The antibodies recognizing the adrenal specific enzyme 21-hydroxylase are important markers of adrenal autoimmunity. This can be the case if the disease presents as Addison’s disease or as part of the autoimmune polyglandular syndrome together with diabetes mellitus type I and thyroid disorders.


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