17OH Progesterone Saliva ELISA Assay Kit

$230.00

Eagle Biosciences 17OH Progesterone Saliva ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of 17OH progesterone concentration in saliva. 17OH Progesterone Saliva ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM023 Categories: , ,

17OH Progesterone Saliva ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 4.9 pg/ml
Dynamic Range: 0 – 1600 pg/ml
Incubation Time: 1.5 hour
Sample Type: Saliva
Sample Size: 50 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

17OH Progesterone (17α-OHP, 17-hydroxy-4-Pregnene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans.

As with cortisol, serum 17α -OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night. In addition, ovarian production of 17α -OHP increases during the luteal phase of the menstrual cycle, leading to serum levels which are several-fold higher than during the follicular phase. The level of 17OHP in saliva (pg/mL) is significantly lower than levels in the general circulation (ng/mL). Progesterone appears to prevent endometrial cancer by regulating the effects of estrogen. Measurement of circulating 17α -OHP levels is a standard tool for clinical assessment of 21-hydroxylase deficiency, the most common cause of congenital adrenal hyperplasia. Due to the decreased activity of 21-hydroxylase, 17α -OHP cannot be efficiently converted to cortisol and, instead, accumulates in large amounts and is shunted into the androgen biosynthetic pathway. 17α -OHP levels are useful in monitoring steroid replacement therapy.

Assay Principle

In the 17OH Progesterone Saliva ELISA Assay Kit, the (antigen) in the sample competes with the antigenic 17OH Progesterone conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti 17OH Progesterone coated on the microplate (solid phase).

After incubation, the bound/free separation is performed by a simple solid-phase washing.Then the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the 17OH Progesterone concentration of in the sample. 17OH Progesterone concentration in the sample is calculated through a calibration curve.

Manual

Product Manual