109 Foods IgG Allergy ELISA

$160.00

The 109 Foods IgG Allergy ELISA is intended for use in the semi-quantitative determination of IgG-mediated food sensitivities of 109 food antigens in human serum and plasma. The Eagle Biosciences IgG Allergy (109 foods) ELISA Assay Kit is intended for research use only and not in diagnostic procedures.

SKU: CNS14M Categories: , ,

109 Foods IgG Allergy ELISA

The 109 Foods IgG Allergy ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-off
Dynamic Range: Negative <8 AU/ml, Borderline 8-12.5 AU/ml, Positive 12.5+
Incubation Time: 1.25 hours
Sample Type: Serum, plasma
Sample Size: 10 µL

Controls Included


Assay Principle

The kit uses a 96-well microtitre plate format. The serum specimen (diluted 1:400) is added to the antigen-coated wells and incubated for 30 minutes to allow food-specific antibodies to bind to the antigens in the microplate wells. After washing, the specifically bound antibodies are detected using goat anti-human IgG conjugated to horseradish peroxidase. After 30 minutes incubation, unbound conjugate is removed by washing, and TMB substrate is added. A blue colour develops in wells in which food-specific antibodies are present and this changes to yellow upon addition of Stop Solution. The absorbance of the yellow solution is then measured in a microplate reader using 450nm or 450/620nm filters.


Specimen Collection and Storage
Serum, plasma or whole blood samples may be used and should be stored at – 20°C for long-term storage. Frozen samples must be mixed well after thawing and prior to testing. Repeated freezing and thawing can affect results. Addition of preservatives to the serum sample may adversely affect the results. Microbially contaminated, heat-treated or specimens containing particulate matter should not be used. Grossly haemolysed, icteric or lipaemic specimens should be avoided.


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Additional Information

Assay Background


Many people exhibit chronic food sensitivity reactions to specific food antigens. Unlike the immediate effects of IgE-mediated allergy, IgG-mediated food sensitivity reactions may take several days to appear. Controlled removal of the problem foods from the patient’s diet will, in many cases, rapidly improve the patient’s condition. General lethargy, weight gain, dermatitis, arthritis and tiredness are associated with food allergies. Irritable bowel syndrome may also be linked to food sensitivity.

Assay Procedure


  1. Add 10µl of Sample to one vial of Diluent (Reagent 1).
  2. Dispense Standards, the Positive Control and the diluted sample into the specified microplate wells.
  3. Incubate for 30 minutes at room temperature.
  4. Wash the wells three times.
  5. Dispense 100µl of Conjugate (Reagent 3) into each well.
  6. Incubate at room temperature for 30 minutes.
  7. Wash the wells four times
  8. Add 100µl of TMB Substrate (Reagent 4) to each well
  9. Incubate at room temperature for 10 minutes
  10. Add 100µl Stop Solution (Reagent 5) to each well
  11. Read the optical density at 450nm (single wavelength) or 450/620nm (dual wavelength).

List of Allergens


109 Foods IgG Allergy ELISA

Manual

Product Manual