109 Foods IgG Allergy ELISA
The 109 Foods IgG Allergy ELISA is For Research Use Only
Size: 1×96 wells
Dynamic Range: Negative <8 AU/ml, Borderline 8-12.5 AU/ml, Positive 12.5+
Incubation Time: 1.25 hours
Sample Type: Serum, plasma
Sample Size: 10 µL
The kit uses a 96-well microtitre plate format. The serum specimen (diluted 1:400) is added to the antigen-coated wells and incubated for 30 minutes to allow food-specific antibodies to bind to the antigens in the microplate wells. After washing, the specifically bound antibodies are detected using goat anti-human IgG conjugated to horseradish peroxidase. After 30 minutes incubation, unbound conjugate is removed by washing, and TMB substrate is added. A blue colour develops in wells in which food-specific antibodies are present and this changes to yellow upon addition of Stop Solution. The absorbance of the yellow solution is then measured in a microplate reader using 450nm or 450/620nm filters.
Specimen Collection and Storage
Serum, plasma or whole blood samples may be used and should be stored at – 20°C for long-term storage. Frozen samples must be mixed well after thawing and prior to testing. Repeated freezing and thawing can affect results. Addition of preservatives to the serum sample may adversely affect the results. Microbially contaminated, heat-treated or specimens containing particulate matter should not be used. Grossly haemolysed, icteric or lipaemic specimens should be avoided.