Eagle bio ELISA data analyzer

online ELISA assay results calculator

Perform curve fitting with Assayfit Pro using this page. You can use this service for free 10 times each day. Click here to read the terms for use and distribution.
Alternatively use the Microsoft Excel add-in to perform the calculation.
For Research Use Only. Not for use in diagnostic procedures.

Paste ELISA results here

Enter your ELISA data in the spreadsheet like table below, you can copy and paste from Excel. Optional: you can subtract blank values or normalize, select this from the options menu.
Options

ELISA layout and sample IDs

Select a region and click the B, C, S, CAL buttons to fill the region with blank, control, sample or calibrator values. You can use the S>, Rv buttons to specify the direction of the filling process and the number of replicates. Sample IDs are kept on your local computer and are not sent over the internet.

Sample dilution

Options

Curve fit input values

You can copy the values from Excel, enter them manually or use the 'Cals from layout' button to copy all calibrator values marked with CAL- from the layout.

Curve fit settings

Function

Server

Key

Run ID

ELISA data analysis results

Fit Summary

Calibration curve

Results

Sample ID

Response

Concentration

Dilution

Conc x Dilution

Fit output